Triticale flour films added with bacteriocin-like substance (BLIS) for active food packaging applications

Abstract

Active triticale flour films were obtained by adding bacteriocin-like substances (BLIS) produced by Enterococcus faecium ES216 with antimicrobial activity against Listeria innocua ATCC33090. Active and control films were characterized. The incorporation of the active fractions did not significantly affect the density, moisture content, water vapor permeability and puncture strength of films. Solubility, color and opacity were dependent on the fraction incorporated into the films. Activated films maintain the antilisterial activity until 45 days of evaluation. Also, active and control triticale films were used to obtain heat-sealed pouches to package inoculated cheese slices under controlled conditions. Active triticale packaging could reduce the counts of Listeria in 3 log comparing to control after 15 days of storage. These results suggest that triticale flour films activated with this BLIS could provide an alternative strategy for active food packaging applications.