Artículo
High-level production of Bacillus cereus phospholipase C in Corynebacterium glutamicum
Ravasi, Pablo
; Braia, Mauricio Javier
; Eberhardt, Maria Florencia
; Elena, Claudia Edicht
; Cerminati, Sebastián
; Peirú, Salvador
; Castelli, Maria Eugenia
; Menzella, Hugo Gabriel
Fecha de publicación:
12/2015
Editorial:
Elsevier Science
Revista:
Journal of Biotechnology
ISSN:
0168-1656
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Enzymatic oil degumming (removal of phospholipids) using phospholipase C (PLC) is a well-established and environmentally friendly process for vegetable oil refining. In this work, we report the production of recombinant Bacillus cereus PLC in Corynebacterium glutamicum ATCC 13869 in a high cell density fermentation process and its performance in soybean oil degumming. A final concentration of 5.5 g/L of the recombinant enzyme was achieved when the respective gene was expressed from the tac promoter in a semi-defined medium. After treatment with trypsin to cleave the propeptide, the mature enzyme completely hydrolyzed phosphatidylcholine and phosphatidylethanolamine, which represent 70% of the phospholipids present in soybean oil. The results presented here show the feasibility of using B. cereus PLC for oil degumming and provide a manufacturing process for the cost effective production of this enzyme.
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Colecciones
Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos(IPROBYQ)
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Citación
Ravasi, Pablo; Braia, Mauricio Javier; Eberhardt, Maria Florencia; Elena, Claudia Edicht; Cerminati, Sebastián; et al.; High-level production of Bacillus cereus phospholipase C in Corynebacterium glutamicum; Elsevier Science; Journal of Biotechnology; 216; 12-2015; 142-148
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