Artículo
Metaprofiling of the bacterial community in sorghum silages inoculated with lactic acid bacteria
Puntillo, Melisa Anahí
; Peralta, Guillermo Hugo
; Bürgi Fissolo, María de Los Milagros
; Huber, Maria Paula
; Gaggiotti, Mónica del Carmen; Binetti, Ana Griselda
; Vinderola, Celso Gabriel
Fecha de publicación:
10/2022
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Journal of Applied Microbiology
ISSN:
1364-5072
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Aims: To characterize the fermentation process and bacterial diversity of sorghum silage inoculated with Lactiplantibacillus plantarum LpAv, Pediococcus pentosaceus PpM and Lacticaseibacillus paracasei LcAv. Methods and Results: Chopped sorghum was ensiled using the selected strains. Physicochemical parameters (Ammonia Nitrogen/Total Nitrogen, Dry Matter, Crude Protein, Acid Detergent Fibre, Neutral Detergent Fibre, Acid Detergent Lignin, Ether Extract and Ashes), bacterial counts, cell cytometry and 16sRNA sequencing were performed to characterize the ensiling process and an animal trial (BALB/c mice) was conducted in order to preliminary explore the potential of sorghum silage to promote animal gut health. After 30 days of ensiling, the genus Lactobacillus comprised 68.4 ± 2.3% and 73.5 ± 1.8% of relative abundance, in control and inoculated silages respectively. Richness (Chao1 index) in inoculated samples, but not in control silages, diminished along ensiling, suggesting the domination of fermentation by the inoculated LAB. A trend in conferring enhanced protection against Salmonella infection was observed in the mouse model used to explore the potential to promote gut health of sorghum silage. Conclusions: The LAB strains used in this study were able to dominate sorghum fermentation. Significance and Impact of the Study: This is the first report using metaprofiling of 16sRNA to characterize sorghum silage, showing a microbiological insight where resident and inoculated LAB strains overwhelmed the epiphytic microbiota, inhibiting potential pathogens of the genus Klebsiella.
Palabras clave:
INOCULANTS
,
L. PARACASEI
,
L. PLANTARUM
,
NGS
,
P. PENTOSACEUS
,
PROFILING
,
SILAGE
,
SORGHUM
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INLAIN)
Articulos de INST.DE LACTOLOGIA INDUSTRIAL
Articulos de INST.DE LACTOLOGIA INDUSTRIAL
Citación
Puntillo, Melisa Anahí; Peralta, Guillermo Hugo; Bürgi Fissolo, María de Los Milagros; Huber, Maria Paula; Gaggiotti, Mónica del Carmen; et al.; Metaprofiling of the bacterial community in sorghum silages inoculated with lactic acid bacteria; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 133; 4; 10-2022; 2375-2389
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