Supercritical CO2 extraction of bioactive compounds from radish leaves: Yield, antioxidant capacity and cytotoxicity

Abstract

Supercritical Fluid Extraction by using CO2 can be employed as an alternative to conventional methods for extracting functional compounds. Overall extractions curves for extractions of bioactive compounds from radish leaves were performed using scCO2 adding ethanol as co-solvent. Yield, total phenolic compounds, total flavonoids and antioxidant capacity were determined for each extract. The best results were obtained at 35 °C/400 bar and 40 °C/400 bar, with values of total phenolics 1375 mg GAE/100 g d.m. and 1455 mg GAE/100 g d.m., respectively. Antioxidant capacity by DPPH values were 359 mg TE/100 g d.m. (35 °C/400 bar) and 403 mg TE/100 g d.m. (40 °C/400 bar). Maturation of dendritic cells was not promoted being these extracts promising and interesting sources of antioxidative and anti-inflammatory agents for future uses in the pharmaceutical and alimentary industries.