Artículo
Amaranth peptides decreased the activity and expression of cellular tissue factor on LPS activated THP-1 human monocytes
Sabbione, Ana Clara
; Luna Vital, Diego Armando; Scilingo, Adriana Alicia; Añon, Maria Cristina
; González de Mejía, María Elvira
Fecha de publicación:
07/2018
Editorial:
Royal Society of Chemistry
Revista:
Food and Function
ISSN:
2042-650X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The effect of amaranth peptides on the activity and expression of tissue factor (TF) on THP-1 activated cells was evaluated in vitro. An active anticoagulant peptide fraction (AF) was found to inhibit TF expression (IC50 = 0.39 mg mL-1) and activity. Immunocytochemical fluorescence confocal microscopy analysis showed that treated monocytes decreased TF membrane translocation by 49.0% and increased two-fold in nuclei compared to a positive control, indicating a decrease of active TF to initiate the coagulation cascade. Moreover, a cytokine array suggested that the AF mechanism of action implied the inhibition of the NF-κB pathway. Expression of MIP-3α, interleukin-1β, interleukin-1α, TARC, pentaxin 3, and PDGF-AA cytokines was highly suppressed by AF peptides, producing reductions of 78.8%, 61.8%, 54.1%, 42.6%, 37.9% and 37.8%, respectively, compared to a positive control. The results suggest a potential mechanism for the antithrombotic and anti-inflammatory effect of AF, by showing that amaranth peptides play a negative feedback regulatory role over the NF-κB pathway. In this research, we link for the first time the immunomodulatory activity of amaranth peptides with the inhibition of TF expression and therefore their antithrombotic potential.
Palabras clave:
AMARANTH BIOACTIVE PEPTIDES
,
TISSUE FACTOR
,
THROMBOSIS
,
THP-1 MONOCYTES
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CIDCA)
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Citación
Sabbione, Ana Clara; Luna Vital, Diego Armando; Scilingo, Adriana Alicia; Añon, Maria Cristina; González de Mejía, María Elvira; Amaranth peptides decreased the activity and expression of cellular tissue factor on LPS activated THP-1 human monocytes; Royal Society of Chemistry; Food and Function; 9; 7; 7-2018; 3823-3834
Compartir
Altmétricas