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Artículo

Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation

Ochnio, María Emilia; Martínez, Karina DafneIcon ; Allievi, Mariana CaludiaIcon ; Palavecino Ruiz, Marcos DanielIcon ; Martínez, Karina DafneIcon ; Perez, Oscar EdgardoIcon
Fecha de publicación: 02/2018
Editorial: MDPI
Revista: Polymers
ISSN: 2073-4360
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Químicas

Resumen

Isolated 7S and 11S globulins obtained from defeated soy flour were complexated with folic acid (FA) in order to generate nano-carriers for this important vitamin in human nutrition. Fluorescence spectroscopy and dynamic light scattering were applied to follow the nano-complexes formation and for their characterization. Fluorescence experimental data were modeled by the Stern-Volmer and a modified double logarithm approach. The results obtained confirmed static quenching. The number of binding sites on the protein molecule was ~1. The values obtained for the binding constants suggest a high affinity between proteins and FA. Particle size distribution allowed to study the protein aggregation phenomenon induced by FA bound to the native proteins. Z-average manifested a clear trend to protein aggregation. 11S-FA nano-complexes resulted in more polydispersity. ζ-potential of FA nano-complexes did not show a remarkable change after FA complexation. The biological activity of nano-complexes loaded with FA was explored in terms of their capacity to enhance the biomass formation of Lactobacillus casei BL23. The results concerning to nano-complexes inclusion in culture media showed higher bacterial growth. Such a result was attributed to the entry of the acid by the specific receptors concomitantly by the peptide receptors. These findings have technological impact for the use of globulins-FA based nano-complexes in nutraceutical, pharmaceutical and food industries.
Palabras clave: 7s And 11s Globulins , Folic Acid , Lactobacillus Casei Bl23 , Nano-Complexation
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/60115
DOI: http://dx.doi.org/10.3390/polym10020149
URL: http://www.mdpi.com/2073-4360/10/2/149
Colecciones
Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos(OCA CIUDAD UNIVERSITARIA)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA CIUDAD UNIVERSITARIA
Citación
Ochnio, María Emilia; Martínez, Karina Dafne; Allievi, Mariana Caludia; Palavecino Ruiz, Marcos Daniel; Martínez, Karina Dafne; et al.; Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation; MDPI ; Polymers; 10; 2; 2-2018; 1-21
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