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Artículo

Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives

Montel Mendoza, María GabrielaIcon ; Ale, Cesar EmmanuelIcon ; Nader, Maria Elena FatimaIcon ; Pasteris, Sergio EnriqueIcon
Fecha de publicación: 24/08/2015
Editorial: Omics International
Revista: Journal of Bioprocessing & Biotechniques
ISSN: 2155-9821
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Enterococcus gallinarum CRL 1826 isolated from an American bullfrog (Lithobates catesbeianus) skin inhibits the growth of Citrobacter freundii, Pseudomonas aeruginosa (bullfrog pathogens) and Listeria monocytogenes by a synergistic effect between organic acids and a bacteriocin-like molecule. This bacteriocin, named enterocin CRL 1826, showed a proteinaceous nature, heat stability and polar characteristics. Its production followed kinetics of primary metabolites synthesis reaching a maximum of 61,400 AU/mL. The minimum inhibitory and minimumbactericidal concentrations were 2,640 and 5,280 AU/mL, respectively, against L. monocytogenes. The addition of 120,000 AU/mL of enterocin to growing L. monocytogenes and Gram-negative (P. aeruginosa and C. freundii) bacteria showed bactericidal and bacteriostatic effects, respectively. However, enterocin derived-peptides had bactericidal effect only against Gram-negatives.Enterocin produced cell envelope damages and efflux of citosolic content on L. monocytogenes, while enterocin derived-peptides showed granulation and contraction of cytoplasm material on P. aeruginosa and increase in theperiplasmic space and empty cells appearance on C. freundii.Enterocin CRL 1826 is the first bacteriocin described for E. gallinarum from raniculture. It could be used as a biopreservative while the derived-peptides represent an alternative to control multi-drug resistant Gram-negatives.The antimicrobial spectrum and the stability of enterocin and its derived-peptides indicate that they could be applied in different biotechnological areas.
Palabras clave: Enterococcus Gallinarum , Raniculture , Bacteriocin , Probiotics , Biopreservatives
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/28259
DOI: http://dx.doi.org/10.4172/2155-9821.1000250
URL: https://www.omicsonline.org/open-access/characterization-of-a-bacteriocin-produc
Colecciones
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos(CERELA)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Citación
Montel Mendoza, María Gabriela; Ale, Cesar Emmanuel; Nader, Maria Elena Fatima; Pasteris, Sergio Enrique; Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives; Omics International; Journal of Bioprocessing & Biotechniques; 5; 8; 24-8-2015; 1-9;1000250
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