Artículo
Impairment of Listeria monocytogenes biofilm developed on industrial surfaces by Latilactobacillus curvatus CRL1579 bacteriocin
Melian, Constanza Victoria
; Ploper, Diego
; Chehin, Rosana Nieves
; Vignolo, Graciela Margarita
; Castellano, Patricia Haydee





Fecha de publicación:
08/2024
Editorial:
Academic Press Ltd - Elsevier Science Ltd
Revista:
Food Microbiology
ISSN:
0740-0020
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The effect of lactocin AL705, bacteriocin produced by Latilactobacillus (Lat.) curvatus CRL1579 against Listeria biofilms on stainless steel (SS) and polytetrafluoroethylene (PTFE) coupons at 10 ◦C was investigated. L. monocytogenes FBUNT showed the greatest adhesion on both surfaces associated to the hydrophobicity of cell surface. Partially purified bacteriocin (800 UA/mL) effectively inhibited L. monocytogenes preformed biofilm through displacement strategy, reducing the pathogen by 5.54 ± 0.26 and 4.74 ± 0.05 log cycles at 3 and 6 days, respectively. The bacteriocin-producer decreased the pathogen biofilm by ~2.84 log cycles. Control and Bac− treated samples reached cell counts of 7.05 ± 0.18 and 6.79 ± 0.06 log CFU/cm2 after 6 days of incubation. Confocal scanning laser microscopy (CLSM) allowed visualizing the inhibitory effect of lactocin AL705 on L. monocytogenes preformed biofilms under static and hydrodynamic flow conditions. A greater effect of the bacteriocin was found at 3 days independently of the surface matrix and pathogen growth conditions at 10 ◦C. As a more realistic approach, biofilm displacement strategy under continuous flow conditions showed a significant loss of biomass, mean thickness and substratum coverage of pathogen biofilm. These findings highlight the anti- biofilm capacity of lactocin AL705 and their potential application in food industries.
Palabras clave:
Lactocin
,
biofilm
,
Listeria monocytogenes
,
Confocal microscopy
Archivos asociados
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Identificadores
Colecciones
Articulos (IMMCA)
Articulos de INSTITUTO DE INVESTIGACIONES EN MEDICINA MOLECULAR Y CELULAR APLICADA DEL BICENTENARIO
Articulos de INSTITUTO DE INVESTIGACIONES EN MEDICINA MOLECULAR Y CELULAR APLICADA DEL BICENTENARIO
Articulos(CERELA)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Citación
Melian, Constanza Victoria; Ploper, Diego; Chehin, Rosana Nieves; Vignolo, Graciela Margarita; Castellano, Patricia Haydee; Impairment of Listeria monocytogenes biofilm developed on industrial surfaces by Latilactobacillus curvatus CRL1579 bacteriocin; Academic Press Ltd - Elsevier Science Ltd; Food Microbiology; 121; 8-2024; 1-10
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