Evento
Characterization of ten newly isolated phages against the foodborne pathogen Shigella flexneri
Capra, María Luján
; Tomat, David Damian
; Gonzalez, A.; Aquili, Virginia Daniela; Casabonne, Cecilia; Quiberoni, Andrea del Lujan
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Tipo del evento:
Congreso
Nombre del evento:
VIII Congreso Internacional de Ciencia y Tecnología de Alimentos
Fecha del evento:
04/10/2022
Institución Organizadora:
Ministerio de Ciencia y Tecnología;
Título del Libro:
Libro de Resúmenes: VIII Congreso Internacional de Ciencia y Tecnología de los Alimentos
Editorial:
Ministerio de Ciencia y Tecnología
ISBN:
978-987-47203-5-1
Idioma:
Inglés
Clasificación temática:
Resumen
Shigella is one of the most important waterborne and foodborne bacterial pathogens in the world. The species most commonly isolated from shigellosis patients is Shigella flexneri, which is found predominantly in developing countries like Argentina. Shigellosis is considered to be the most important cause of bloody diarrhea worldwide and is one of the main causes of children’s morbidity and mortality in Argentina. Ten (10) bacteriophages (AShi, Shi3, Shi22, Shi30, Shi33, Shi34, Shi40, Shi88, Shi93, and Shi113) with lytic activity against Shigella flexneri strains were tested for their resistance to physicochemical conditions found in food matrices. Phage viability and activity were assessed at different temperatures, pH values and NaCl concentrations. In addition, challenge tests with each individual phage against S. flexneri ATCC12022 were conducted to evaluate their use as biocontrol agents. Although phages showed high resistance up to 60°C, Shi30, Shi33 and Shi93 showed higher thermal resistance since phage particles were detected after a 1-h incubation at 70°C. The ten phages tolerated pH treatments well, being more resistant to alkaline conditions - up to pH 11 - since the viability and activity of phages were completely abolished only at the extreme pH values tested, namely pH 2 and 12. The NaCl concentrations evaluated showed no significant influence on phage counts. However, the activity of two phages was slightly affected only at the highest concentration tested (10%). Furthermore, challenge tests against ATCC12022 resulted in significant CFU mL-1 reductions. Results showed that the phages evaluated were resistant i) to a wide range of temperatures, ii) at pH values from 3 to 11, and iii) at all the concentrations of NaCl tested. Therefore, the phages evaluated in this study could be used in several food matrices since they are viable and active in a wide range of environmental conditions.This research was supported by the Agencia Nacional de Promoción Científica y Tecnológica (Project PICT 2017-0101) of Argentina.
Palabras clave:
BACTERIOPHAGE
,
PHAGE VIABILITY
,
LYTIC ACTIVITY
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Eventos de INST.DE LACTOLOGIA INDUSTRIAL
Eventos de INST.DE LACTOLOGIA INDUSTRIAL
Citación
Characterization of ten newly isolated phages against the foodborne pathogen Shigella flexneri; VIII Congreso Internacional de Ciencia y Tecnología de Alimentos; Córdoba; Argentina; 2022; 1-3
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