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Artículo

Betanin loaded nanocarriers based on quinoa seed 11S globulin. Impact on the protein structure and antioxidant activity

Martínez, María JimenaIcon ; Velázquez Duarte, FranciscoIcon ; Burrieza, Hernán PabloIcon ; Martínez, Karina DafneIcon ; Domínguez Rubio, Ana PaulaIcon ; dos Santos Ferreira, Cristina; del Pilar Buera, María; Perez, Oscar EdgardoIcon
Fecha de publicación: 02/2019
Editorial: Elsevier
Revista: Food Hydrocolloids
ISSN: 0268-005X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Nanotecnología

Resumen

The objective of the present contribution was to design and characterize betanin (Bt) loaded in a nanovehicle of 11S quinoa seed protein. 11S was isolated from quinoa seed floor. Protein purification was performed by Size-Exclusion Chromatography. MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time-Of- Flight) analysis confirmed the identity of 11S. Nanocarriers (11S-Bt) were generated at pH 8 at different ionic strength. Globulin intrinsic fluorescence spectra showed a quenching effect exerted by Bt, demonstrating in turn protein-bioactive interaction. Stern-Volmer and Scatchard models application confirmed static quenching and allow obtaining parameters that described 11S and betanincomplexation process. Bt-11S globulin interactions seem to be more probably of physical type. Protein solubility was increased after complexation with Bt. 11S betanin-loaded nanocarrier showed additive effect in terms of both, antiradical or reducing power capacity in comparison to Bt as evaluated by two methods, 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and by ferric reducing antioxidant power (FRAP). Interestingly 11S globulin quaternary structure was modified by the bioactive, experimenting hexamer dissociation. This nanocolloid could have the potentiality to exert the Bt controlled delivery for pharmaceutical and nutraceutical products. Bt could also be protected from light and oxygen in such systems.
Palabras clave: 11S quinoa globulin , Betanin , Antioxidant activity , Nanocarrier , 11S quinoa globulin , Betanin
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/121693
URL: https://linkinghub.elsevier.com/retrieve/pii/S0268005X18304004
DOI: http://dx.doi.org/10.1016/j.foodhyd.2018.09.016
Colecciones
Articulos(IBBEA)
Articulos de INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos(ITAPROQ)
Articulos de INSTITUTO DE TECNOLOGIA DE ALIMENTOS Y PROCESOS QUIMICOS
Citación
Martínez, María Jimena; Velázquez Duarte, Francisco; Burrieza, Hernán Pablo; Martínez, Karina Dafne; Domínguez Rubio, Ana Paula; et al.; Betanin loaded nanocarriers based on quinoa seed 11S globulin. Impact on the protein structure and antioxidant activity; Elsevier; Food Hydrocolloids; 87; 2-2019; 880-890
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