Effect of Enterocin CRL35 on Listeria monocytogenes cell membrane

Abstract

The antimicrobial peptide Enterocin CRL35, a class II bacteriocin, produces at high concentrations (8 μg ml-1) localized holes in the wall and cellular membrane of Listeria monocytogenes, reflected in the efflux of macromolecules such as proteins and other ultraviolet-absorbing materials. At lower concentrations (0.5 μg ml-1), neither ultra structural changes nor macromolecules efflux were observed, however potassium and phosphate ions were released, dissipating the proton motive force. As a result the bacteria were killed.