Creating 2D Occupancy Plots Using plot2DO

Abstract

The 2D occupancy (2DO) plot is useful for an initial quality check of MNase-seq data, in order to assess the degree of chromatin digestion, and also for getting insights about the nucleosome organization, as well as about the MNase digestion kinetics.plot2DO is a flexible tool for evaluating the quality of MNase-seq and MNase-ChIP-seq data, and for visualizing the distribution of nucleosomes near the functional regions of the genome.