Evaluation of biomarkers in glioma by immunohistochemistry on paraffin- embedded 3D glioma neurosphere cultures

Abstract

Analysis of protein expression in glioma is relevant for several aspects in the study of its pathology. Numerous proteins have been described asbiomarkers with applications in diagnosis, prognosis, classification, state of tumor progression, and cell differentiation state. These analyses ofbiomarkers are also useful to characterize tumor neurospheres (NS) generated from glioma patients and glioma models. Tumor NS provide avaluable in vitro model to assess different features of the tumor from which they are derived and can more accurately mirror glioma biology. Herewe describe a detailed method to analyze biomarkers in tumor NS using immunohistochemistry (IHC) on paraffin-embedded tumor NS.