Artículo
Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation
Campos, Débora; Coscueta, Ezequiel Ricardo
; Woitovich Valetti, Nadia
; Pastrana Castro, Lorenzo M.; Teixeira, José; Picó, Guillermo Alfredo
; Pintado, Maria Manuela
Fecha de publicación:
02/2019
Editorial:
Elsevier
Revista:
Food Hydrocolloids
ISSN:
0268-005X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A simple method for bromelain extraction from industrial pineapple residues (stems and peels) was developed and optimized through factorial experimental design. The developed methodology, based on precipitation with carrageenan, represents an alternative to the use of organic solvents and inorganic salts (common industrial precipitation) and allows achieving extracts with high bromelain purity. High recovery yield – 80–90% - of active bromelain was obtained for both crude juices (stems and peels) making possible to obtain ca. 0.3 g of bromelain from 100 g of pineapple byproducts using a low polysaccharide concentration (0.2–0.3% w/v).
Palabras clave:
BROMELAIN
,
CARRAGEENAN
,
NATURAL CRUDE JUICES
,
PROTEIN COMPLEX
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Articulos(IPROBYQ)
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Citación
Campos, Débora; Coscueta, Ezequiel Ricardo; Woitovich Valetti, Nadia; Pastrana Castro, Lorenzo M.; Teixeira, José; et al.; Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation; Elsevier; Food Hydrocolloids; 87; 2-2019; 792-804
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