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Artículo

Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells

Prudkin Silva, Cecilia RaquelIcon ; Martinez, Jimena HebeIcon ; Martínez, Karina DafneIcon ; Farías Hermosilla, María EstefaníaIcon ; Coluccio Leskow, FedericoIcon ; Perez, Oscar EdgardoIcon
Fecha de publicación: 01/2018
Editorial: Elsevier Science
Revista: Colloids and Surfaces A: Physicochemical and Engineering Aspects
ISSN: 0927-7757
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Nanotecnología

Resumen

The objective of this contribution was to propose a model that would explain the nanocomplexes formation between Human Recombinant Insulin (I) and a polydisperse Chitosan (CS). Such an objective implied exploring I and CS concentration conditions that allowed the formation of complexes with defined and reproducible submicronic dimensions. I-CS complexes were obtained by mixing I and CS solutions at pH 2 and then increasing the pH up to 6 promoting electrostatic interactions between them. Colloidal stages of I and I-CS nano-complexes formation were characterized by dynamic light scattering (DLS), ζ-potential, solutions flow behavior and absorbance measurements. 1·10−2%, w/w, of CS allowed covering completely the surface protein aggregates constituting core–shell nano-structures of 200 nm, with a ζ-potential of 17,5 mV. Solution dynamic viscosity results kept relation with different stages of nano-complexation process. Biological activity of I-CS complexes was studied in 3T3-L1 cultured fibroblast showing a delayed and sustained activity as compared to free insulin. I-CS nano-complexes could be an alternative for developing a new generation of drugs allowing I protection from the hostile conditions of the body and increasing its absorption. These findings have basic and practical impacts as they could be exploited to exert the controlled release of I in therapeutic formulations by using the I-CS nano-complexes.
Palabras clave: CHITOSAN , INSULIN , MACROMOLECULAR ASSEMBLY , NANO-COMPLEXES
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/90593
DOI: http://dx.doi.org/10.1016/j.colsurfa.2017.10.040
URL: https://www.sciencedirect.com/science/article/pii/S0927775717309391
Colecciones
Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Prudkin Silva, Cecilia Raquel; Martinez, Jimena Hebe; Martínez, Karina Dafne; Farías Hermosilla, María Estefanía; Coluccio Leskow, Federico; et al.; Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells; Elsevier Science; Colloids and Surfaces A: Physicochemical and Engineering Aspects; 537; 1-2018; 425-434
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