Artículo
The production, properties, and applications of thermostable steryl glucosidases
Aguirre, Andres
; Eberhardt, Maria Florencia
; Hails, Guillermo
; Cerminati, Sebastián
; Castelli, Maria Eugenia
; Rasia, Rodolfo Maximiliano
; Paoletti, Luciana Elisa
; Menzella, Hugo Gabriel
; Peirú, Salvador
Fecha de publicación:
03/2018
Editorial:
Springer
Revista:
World Journal of Microbiology
ISSN:
0959-3993
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Extremophilic microorganisms are a rich source of enzymes, the enzymes which can serve as industrial catalysts that can withstand harsh processing conditions. An example is thermostable β-glucosidases that are addressing a challenging problem in the biodiesel industry: removing steryl glucosides (SGs) from biodiesel. Steryl glucosidases (SGases) must be tolerant to heat and solvents in order to function efficiently in biodiesel. The amphipathic nature of SGs also requires enzymes with an affinity for water/solvent interfaces in order to achieve efficient hydrolysis. Additionally, the development of an enzymatic process involving a commodity such as soybean biodiesel must be cost-effective, necessitating an efficient manufacturing process for SGases. This review summarizes the identification of microbial SGases and their applications, discusses biodiesel refining processes and the development of analytical methods for identifying and quantifying SGs in foods and biodiesel, and considers technologies for strain engineering and process optimization for the heterologous production of a SGase from Thermococcus litoralis. All of these technologies might be used for the production of other thermostable enzymes. Structural features of SGases and the feasibility of protein engineering for novel applications are explored.
Palabras clave:
BIOFUELS
,
EXTREMOZYMES
,
STERYL GLUCOSIDASES
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Licencia
Identificadores
Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos(IPROBYQ)
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Citación
Aguirre, Andres; Eberhardt, Maria Florencia; Hails, Guillermo; Cerminati, Sebastián; Castelli, Maria Eugenia; et al.; The production, properties, and applications of thermostable steryl glucosidases; Springer; World Journal of Microbiology; 34; 3; 3-2018; 39-45
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