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Artículo

Differential proteomic analysis of lactic acid bacteria—Escherichia coli O157:H7 Interaction and Its contribution to bioprotection strategies in meat

Orihuel, AlejandraIcon ; Teran, Lucrecia CeciliaIcon ; Renaut, Jenny; Vignolo, Graciela MargaritaIcon ; Almeida, André Martinho de; Saavedra, Maria LucilaIcon ; Fadda, Silvina G.Icon
Fecha de publicación: 05/06/2018
Editorial: Frontiers Research Foundation
Revista: Frontiers in Microbiology
ISSN: 1664-302X
e-ISSN: 1664-302X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología; Otros Tópicos Biológicos

Resumen

Human infection by Enterohemorrhagic Escherichia (E.) coli (EHEC) occurs through the ingestion of contaminated foods such as milk, vegetable products, water-based drinks, and particularly minced meats. Indeed EHEC is a pathogen that threatens public health and meat industry. The potential of different Lactic Acid Bacteria (LAB) strains to control EHEC in a meat-based medium was evaluated by using a simple and rapid method and by analyzing the growth kinetics of co-cultures (LAB-EHEC) in a meat-based medium. The activity of LAB toward EHEC in co-cultures showed variable inhibitory effect. Although, LAB were able to control EHEC, neither the produced acid nor bacteriocins were responsible of the inhibition. The bacteriocinogenic Enteroccus (Ent.) mundtii CRL35 presented one of the highest inhibition activities. A proteomic approach was used to evaluate bacterial interaction and antagonistic mechanisms between Ent. mundtii and EHEC. Physiological observations, such as growth kinetics, acidification ability and EHEC inhibitory potential were supported by the proteomic results, demonstrating significant differences in protein expression in LAB: (i) due to the presence of the pathogen and (ii) according to the growth phase analyzed. Most of the identified proteins belonged to carbohydrate/amino acid metabolism, energy production, transcription/translation, and cell division. These results contribute to the knowledge of competition strategies used by Ent. mundtii during its co-culture with EHEC setting new perspectives for the use of LAB to control this pathogen in meat.
Palabras clave: BACTERIAL INTERACTION , BIOPROTECTIVE CULTURES , ENTEROHEMORRHAGIC ESCHERICHIA COLI (EHEC) , LACTIC ACID BACTERIA (LAB) , MEAT SAFETY , PROTEOMICS
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/89402
DOI: https://dx.doi.org/10.3389/fmicb.2018.01083
URL: https://www.frontiersin.org/articles/10.3389/fmicb.2018.01083
Colecciones
Articulos(CERELA)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Citación
Orihuel, Alejandra; Teran, Lucrecia Cecilia; Renaut, Jenny; Vignolo, Graciela Margarita; Almeida, André Martinho de; et al.; Differential proteomic analysis of lactic acid bacteria—Escherichia coli O157:H7 Interaction and Its contribution to bioprotection strategies in meat; Frontiers Research Foundation; Frontiers in Microbiology; 9; 5-6-2018
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