Artículo
Hydrolysis of lactose from cheese whey using a reactor with beta-galactosidase enzyme immobilized on a commercial UF membrane
Fecha de publicación:
10/2013
Editorial:
Versita
Revista:
Chemical And Process Engineering-inzynieria Chemiczna I Procesowa
ISSN:
2300-1925
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In this study, ß-galactosidase enzyme from Kluyveromyces fragilis was immobilised on a commercial polyethersulfone membrane surface, 10 kDa cut-off. An integrated process, concerning the simultaneous hydrolysis–ultrafiltration of whey lactose was studied and working conditions have been fixed at 55°C and pH 6.9, the same conditions that are used for the industrial process of protein concentration. For the immobilisation, best results were obtained using 5% (v/v) of glutaraldehyde solution and 0.03 M galactose; the total activity recovery coefficient (TARC) was 44.2%. The
amount of immobilised enzyme was 12.49 mg with a total activity of 86.3 LAU at 37°C, using 5% (w/v) lactose solution in phosphate buffer (100 mM pH 6.9). The stability of the immobilised enzyme was approximately 585 fold higher in comparison with the stability of free enzyme. Multipoint covalent immobilisation improves the stability of the enzyme, thereby enhancing the decision to use the membrane as a filtering element and support for the enzyme immobilisation.
Palabras clave:
Lactose
,
Enzymatic Hydrolysis
,
Membrane Bioreactor
,
Whey
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Articulos(INCAPE)
Articulos de INST.DE INVEST.EN CATALISIS Y PETROQUIMICA "ING. JOSE MIGUEL PARERA"
Articulos de INST.DE INVEST.EN CATALISIS Y PETROQUIMICA "ING. JOSE MIGUEL PARERA"
Articulos(INTEC)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Citación
Regenhardt, Silvina Andrea; Mammarella, Enrique José; Rubiolo, Amelia Catalina; Hydrolysis of lactose from cheese whey using a reactor with beta-galactosidase enzyme immobilized on a commercial UF membrane; Versita; Chemical And Process Engineering-inzynieria Chemiczna I Procesowa; 34; 3; 10-2013; 375-385
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