Artículo
Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
de Azevedo, M. S. P.; Santos Rocha, C.; Pereira, V. B.; de Oliveira Junior, A. .; de Sousa, C. S.; Azevedo, V.; Leblanc, Jean Guy Joseph
; Chatel, J. M.; Miyoshi, A.
Fecha de publicación:
28/12/2015
Editorial:
Fundacao de Pesquisas Científicas de Riberao Preto
Revista:
Genetics and Molecular Research
ISSN:
1676-5680
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A hemolytic assay showed that the recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing strain of L. lactis may be used as an alternative tool in DNA vaccination against a number of infectious diseases or in cancer therapy.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CERELA)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Citación
de Azevedo, M. S. P.; Santos Rocha, C.; Pereira, V. B.; de Oliveira Junior, A. .; de Sousa, C. S.; et al.; Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination; Fundacao de Pesquisas Científicas de Riberao Preto; Genetics and Molecular Research; 14; 4; 28-12-2015; 18485-18493
Compartir
Altmétricas