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dc.contributor.author
de Azevedo, M. S. P.  
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Santos Rocha, C.  
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Pereira, V. B.  
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de Oliveira Junior, A. .  
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de Sousa, C. S.  
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Azevedo, V.  
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Leblanc, Jean Guy Joseph  
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Chatel, J. M.  
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Miyoshi, A.  
dc.date.available
2019-06-24T18:32:55Z  
dc.date.issued
2015-12-28  
dc.identifier.citation
de Azevedo, M. S. P.; Santos Rocha, C.; Pereira, V. B.; de Oliveira Junior, A. .; de Sousa, C. S.; et al.; Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination; Fundacao de Pesquisas Científicas de Riberao Preto; Genetics and Molecular Research; 14; 4; 28-12-2015; 18485-18493  
dc.identifier.issn
1676-5680  
dc.identifier.uri
http://hdl.handle.net/11336/78738  
dc.description.abstract
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A hemolytic assay showed that the recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing strain of L. lactis may be used as an alternative tool in DNA vaccination against a number of infectious diseases or in cancer therapy.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Fundacao de Pesquisas Científicas de Riberao Preto  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Lactic Acid Bacteria  
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Lactococcus Lactis  
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Listeria Monocytogenes  
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Listeriolysin O  
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Mutated Internalin A  
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Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-06-03T14:35:51Z  
dc.journal.volume
14  
dc.journal.number
4  
dc.journal.pagination
18485-18493  
dc.journal.pais
Brasil  
dc.journal.ciudad
Ribeirao Preto  
dc.description.fil
Fil: de Azevedo, M. S. P.. Universidade Federal de Minas Gerais; Brasil  
dc.description.fil
Fil: Santos Rocha, C.. Universidade Federal de Minas Gerais; Brasil  
dc.description.fil
Fil: Pereira, V. B.. Universidade Federal de Minas Gerais; Brasil  
dc.description.fil
Fil: de Oliveira Junior, A. .. Universidade Federal de Minas Gerais; Brasil  
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Fil: de Sousa, C. S.. Universidade Federal de Minas Gerais; Brasil  
dc.description.fil
Fil: Azevedo, V.. Universidade Federal de Minas Gerais; Brasil  
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Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina  
dc.description.fil
Fil: Chatel, J. M.. Institut National de la Recherche Agronomique; Francia  
dc.description.fil
Fil: Miyoshi, A.. Universidade Federal de Minas Gerais; Brasil  
dc.journal.title
Genetics and Molecular Research  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4238/2015.December.23.36  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.geneticsmr.com/articles/5859  

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