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Artículo

Efficient mannitol production by wild-type Lactobacillus reuteri CRL 1101 is attained at constant pH using a simplified culture medium

Ortiz, María EugeniaIcon ; Raya, Raul RicardoIcon ; Mozzi, Fernanda BeatrizIcon
Fecha de publicación: 18/10/2015
Editorial: Springer
Revista: Applied Microbiology and Biotechnology
ISSN: 0175-7598
e-ISSN: 1432-0614
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biotecnología Industrial

Resumen

Mannitol is a natural polyol with multiple industrial applications. In this work, mannitol production by Lactobacillus reuteri CRL 1101 was studied at free- and controlled-pH (6.0–4.8) fermentations using a simplified culture medium containing yeast and beef extracts and sugarcane molasses. The activity of mannitol 2-dehydrogenase (MDH), the enzyme responsible for mannitol synthesis, was determined. The effect of the initial biomass concentration was further studied. Mannitol production (41.5 ± 1.1 g/l), volumetric productivity (QMtl 1.73 ± 0.05 g/l h), and yield (YMtl 105 ± 11 %) were maximum at pH 5.0 after 24 h while the highest MDH activity (1.66 ± 0.09 U/mg protein) was obtained at pH 6.0. No correlation between mannitol production and MDH activity was observed when varying the culture pH. The increase (up to 2000-fold) in the initial biomass concentration did not improve mannitol formation after 24 h although a 2-fold higher amount was produced at 8 h using 1 or 2 g cell dry weight/l comparing to the control (0.001 g cell dry weight/l). Finally, mannitol isolation under optimum fermentation conditions was achieved. The mannitol production obtained in this study is the highest reported so far by a wild-type L. reuteri strain and, more interestingly, using a simplified culture medium.
Palabras clave: Fermentation , Lactic Acid Bacteria , Polyol , Sugar Alcohol , Sugarcane Molasses
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/59013
DOI: http://dx.doi.org/10.1007/s00253-015-6730-y
URL: https://link.springer.com/article/10.1007%2Fs00253-015-6730-y
Colecciones
Articulos(CERELA)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Citación
Ortiz, María Eugenia; Raya, Raul Ricardo; Mozzi, Fernanda Beatriz; Efficient mannitol production by wild-type Lactobacillus reuteri CRL 1101 is attained at constant pH using a simplified culture medium; Springer; Applied Microbiology and Biotechnology; 99; 20; 18-10-2015; 8717-8729
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