Artículo
N-acetylcysteine, an antioxidant with anti-adipogenic effect on adipocytes
Pieralisi, Azul; Soto, Daniela; Gabrielli, Matias
; Martini, Claudia Noemí; Diz, Virginia Emilse; Rovner, Federico; Vila, Maria del Carmen
; Calvo, Juan Carlos
; Guerra, Liliana Noemi
Fecha de publicación:
07/2017
Editorial:
Elsevier Science Inc
Revista:
Free Radical Biology and Medicine
ISSN:
0891-5849
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Reports about antioxidant effect in obesity are contradictory. We showed that N-acetylcysteine (NAC) inhibits cellular lipid accumulation during adipocyte differentiation, through the inhibition of adipogenic transcriptor factors expression, such as PPARγ and, MAPKs phosphorylation (Pieralisi et al,Redox Biol 2016; Soto el al,Redox Rep 2016). Here we evaluated NAC on fully differentiated cells (3T3-L1 adipocytes: AC). Treatments with 0.01 to 5 mM NAC, included in culture media for 5 days, were not toxic. 5mM NAC treatment on AC (ACN) provoked a decrease of 60% in cellular Triglycerides (Tg) content (1.22+0.09 gTg/g protein [AC] vs 0.49+0.03 gTg/g protein [ACN], p<0.05). We evaluated Oil-Red-O stained lipids content in ACN comparing to AC, which is set to 100 (100+4 [AC] vs 80+2 [ACN] arbitrary units (AU), p< 0.05), lipid protein perilipin (Pl) mRNA levels (Pl/ Rplp0:100+13 [AC] vs 72+9 [ACN] AU, p< 0.05) and PPARγ: protein expression (PPARγ/GAPDH:100+6 [AC] vs 70+4 [ACN]AU; p< 0.05) and mRNA levels (PPARγ/Rplp0:100+7 [AC] vs 74+14 [ACN]AU, p<0.05). These NAC treatments produced a decrease of 20 ? 30%, suggesting that NAC could inhibit new lipid production. We developed liposome of 100nm diameter (LIP) including 5mM NAC, as better tool for NAC administration. Phase transition enthalpy decreased from 3.86 J/g in LIP to 3.25 J/g in LIP+NAC, indicating NAC incorporation.
Palabras clave:
Obesity
,
Antioxidant
,
Adipocyte
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Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Pieralisi, Azul; Soto, Daniela; Gabrielli, Matias; Martini, Claudia Noemí; Diz, Virginia Emilse; et al.; N-acetylcysteine, an antioxidant with anti-adipogenic effect on adipocytes; Elsevier Science Inc; Free Radical Biology and Medicine; 108; 7-2017; 48-49
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