Artículo
Development of a highly efficient oil degumming process using a novel phosphatidylinositol-specific phospholipase C enzyme
Cerminati, Sebastián
; Eberhardt, Maria Florencia
; Elena, Claudia E.; Peirú, Salvador
; Castelli, Maria Eugenia
; Menzella, Hugo Gabriel
Fecha de publicación:
06/2017
Editorial:
Springer
Revista:
Applied Microbiology and Biotechnology
ISSN:
0175-7598
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Enzymatic degumming using phospholipase C (PLC) enzymes may be used in environmentally friendly processes with improved oil recovery yields. In this work, phosphatidylinositol-specific phospholipase C (PIPLC) candidates obtained from an in silico analysis were evaluated for oil degumming. A PIPLC from Lysinibacillus sphaericus was shown to efficiently remove phosphatidylinositol from crude oil, and when combined with a second phosphatidylcholine and phosphatidylethanolamine-specific phospholipase C, the three major phospholipids were completely hydrolyzed, providing an extra yield of oil greater than 2.1%, compared to standard methods. A remarkably efficient fed-batch Escherichia coli fermentation process producing ∼14 g/L of the recombinant PIPLC enzyme was developed, which may facilitate the adoption of this cost-effective oil-refining process.
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Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(IPROBYQ)
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Citación
Cerminati, Sebastián; Eberhardt, Maria Florencia; Elena, Claudia E.; Peirú, Salvador; Castelli, Maria Eugenia; et al.; Development of a highly efficient oil degumming process using a novel phosphatidylinositol-specific phospholipase C enzyme; Springer; Applied Microbiology and Biotechnology; 101; 11; 6-2017; 4471-4479
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