Artículo
Strain engineering and process optimization for enhancing the production of a thermostable steryl glucosidase in Escherichia coli
Fecha de publicación:
01/2017
Editorial:
Springer Heidelberg
Revista:
Journal of Industrial Microbiology & Biotechnology
ISSN:
1367-5435
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Biodiesels produced from transesterification of vegetable oils have a major problem in quality due to the presence of precipitates, which are mostly composed of steryl glucosides (SGs). We have recently described an enzymatic method for the efficient removal of SGs from biodiesel, based on the activity of a thermostable β-glycosidase from Thermococcus litoralis. In the present work, we describe the development of an Escherichia coli-based expression system and a high cell density fermentation process. Strain and process engineering include the assessment of different promoters to drive the expression of a codon-optimized gene, the co-expression of molecular chaperones and the development of a high cell density fermentation process. A 200-fold increase in the production titers was achieved, which directly impacts on the costs of the industrial process for treating biodiesel.
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Articulos(IPROBYQ)
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Citación
Eberhardt, Maria Florencia; Aguirre, Andres; Menzella, Hugo Gabriel; Peirú, Salvador; Strain engineering and process optimization for enhancing the production of a thermostable steryl glucosidase in Escherichia coli; Springer Heidelberg; Journal of Industrial Microbiology & Biotechnology; 44; 1; 1-2017; 141-147
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