Artículo
Nisin Z produced by lactococcus lactis from bullfrog hatchery is active against litrobacter freundii, a red-leg syndrome related pathogen
Quintana, Gabriel Sebastian
; Niederle, María Virginia
; Minahk, Carlos Javier
; Picariello, Gianluca; Nader, Maria Elena Fatima
; Pasteris, Sergio Enrique
Fecha de publicación:
10/2017
Editorial:
Springer
Revista:
World Journal of Microbiology
ISSN:
0959-3993
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Lactococcus lactis subsp. lactis CRL 1584 isolated from a bullfrog hatchery produces a bacteriocin that inhibits both indigenous Citrobacter freundii (a Red-Leg Syndrome related pathogen) and Lactobacillus plantarum, and Listeria monocytogenes as well. Considering that probiotics requires high cell densities and/or bacteriocin concentrations, the effect of the temperature on L. lactis growth and bacteriocin production was evaluated to find the optimal conditions. Thus, the growth rate was maximal at 36 °C, whereas the highest biomass and bacteriocin activity was achieved between 20 and 30 °C and 20–25 °C, respectively. The bacteriocin synthesis was closely growth associated reaching the maximal values at the end of the exponential phase. Since bacteriocins co-production has been evidenced in bacterial genera, a purification of the bacteriocin/s from L. lactis culture supernatants was carried out. The active fraction was purified by cationic-exchange chromatography and then, a RP-HPLC was carried out. The purified sample was a peptide with a 3353.05 Da, a molecular mass that matches nisin Z, which turned out to be the only bacteriocin produced by L. lactis CRL 1584. Nisin Z showed bactericidal effect on C. freundii and L. monocytogenes, which increased in the presence l-lactic acid + H2O2. This is the first report on nisin Z production by L. lactis from a bullfrog hatchery that resulted active on a Gram-negative pathogen. This peptide has potential probiotic for raniculture and as food biopreservative for bullfrog meat.
Palabras clave:
Bacteriocin
,
Biopreservatives
,
Lactococcus Lactis
,
Probiotics
,
Raniculture
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CERELA)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Articulos(CIDCA)
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Quintana, Gabriel Sebastian; Niederle, María Virginia; Minahk, Carlos Javier; Picariello, Gianluca; Nader, Maria Elena Fatima; et al.; Nisin Z produced by lactococcus lactis from bullfrog hatchery is active against litrobacter freundii, a red-leg syndrome related pathogen; Springer; World Journal of Microbiology; 33; 10; 10-2017; 186-198
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