Artículo
Nisin Z produced by lactococcus lactis from bullfrog hatchery is active against litrobacter freundii, a red-leg syndrome related pathogen
Quintana, Gabriel Sebastian
; Niederle, María Virginia
; Minahk, Carlos Javier
; Picariello, Gianluca; Nader, Maria Elena Fatima
; Pasteris, Sergio Enrique





Fecha de publicación:
10/2017
Editorial:
Springer
Revista:
World Journal of Microbiology
ISSN:
0959-3993
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Resumen
Lactococcus lactis subsp. lactis CRL 1584 isolated from a bullfrog hatchery produces a bacteriocin that inhibits both indigenous Citrobacter freundii (a Red-Leg Syndrome related pathogen) and Lactobacillus plantarum, and Listeria monocytogenes as well. Considering that probiotics requires high cell densities and/or bacteriocin concentrations, the effect of the temperature on L. lactis growth and bacteriocin production was evaluated to find the optimal conditions. Thus, the growth rate was maximal at 36 °C, whereas the highest biomass and bacteriocin activity was achieved between 20 and 30 °C and 20–25 °C, respectively. The bacteriocin synthesis was closely growth associated reaching the maximal values at the end of the exponential phase. Since bacteriocins co-production has been evidenced in bacterial genera, a purification of the bacteriocin/s from L. lactis culture supernatants was carried out. The active fraction was purified by cationic-exchange chromatography and then, a RP-HPLC was carried out. The purified sample was a peptide with a 3353.05 Da, a molecular mass that matches nisin Z, which turned out to be the only bacteriocin produced by L. lactis CRL 1584. Nisin Z showed bactericidal effect on C. freundii and L. monocytogenes, which increased in the presence l-lactic acid + H2O2. This is the first report on nisin Z production by L. lactis from a bullfrog hatchery that resulted active on a Gram-negative pathogen. This peptide has potential probiotic for raniculture and as food biopreservative for bullfrog meat.
Palabras clave:
Bacteriocin
,
Biopreservatives
,
Lactococcus Lactis
,
Probiotics
,
Raniculture
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Articulos(CERELA) [831]
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Articulos(CIDCA) [880]
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Articulos(INSIBIO) [490]
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Quintana, Gabriel Sebastian; Niederle, María Virginia; Minahk, Carlos Javier; Picariello, Gianluca; Nader, Maria Elena Fatima; et al.; Nisin Z produced by lactococcus lactis from bullfrog hatchery is active against litrobacter freundii, a red-leg syndrome related pathogen; Springer; World Journal of Microbiology; 33; 10; 10-2017; 186-198
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