Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Nisin Z produced by lactococcus lactis from bullfrog hatchery is active against litrobacter freundii, a red-leg syndrome related pathogen

Quintana, Gabriel SebastianIcon ; Niederle, María VirginiaIcon ; Minahk, Carlos JavierIcon ; Picariello, Gianluca; Nader, Maria Elena FatimaIcon ; Pasteris, Sergio EnriqueIcon
Fecha de publicación: 10/2017
Editorial: Springer
Revista: World Journal of Microbiology
ISSN: 0959-3993
Idioma: Inglés
Tipo de recurso: Artículo publicado

Resumen

Lactococcus lactis subsp. lactis CRL 1584 isolated from a bullfrog hatchery produces a bacteriocin that inhibits both indigenous Citrobacter freundii (a Red-Leg Syndrome related pathogen) and Lactobacillus plantarum, and Listeria monocytogenes as well. Considering that probiotics requires high cell densities and/or bacteriocin concentrations, the effect of the temperature on L. lactis growth and bacteriocin production was evaluated to find the optimal conditions. Thus, the growth rate was maximal at 36 °C, whereas the highest biomass and bacteriocin activity was achieved between 20 and 30 °C and 20–25 °C, respectively. The bacteriocin synthesis was closely growth associated reaching the maximal values at the end of the exponential phase. Since bacteriocins co-production has been evidenced in bacterial genera, a purification of the bacteriocin/s from L. lactis culture supernatants was carried out. The active fraction was purified by cationic-exchange chromatography and then, a RP-HPLC was carried out. The purified sample was a peptide with a 3353.05 Da, a molecular mass that matches nisin Z, which turned out to be the only bacteriocin produced by L. lactis CRL 1584. Nisin Z showed bactericidal effect on C. freundii and L. monocytogenes, which increased in the presence l-lactic acid + H2O2. This is the first report on nisin Z production by L. lactis from a bullfrog hatchery that resulted active on a Gram-negative pathogen. This peptide has potential probiotic for raniculture and as food biopreservative for bullfrog meat.
Palabras clave: Bacteriocin , Biopreservatives , Lactococcus Lactis , Probiotics , Raniculture
Ver el registro completo
 
Archivos asociados
Tamaño: 1.619Mb
Formato: PDF
.
Solicitar
Licencia
info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/50189
URL: http://link.springer.com/10.1007/s11274-017-2353-z
DOI: https://dx.doi.org/10.1007/s11274-017-2353-z
Colecciones
Articulos(CERELA) [831]
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Articulos(CIDCA) [880]
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Articulos(INSIBIO) [490]
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Quintana, Gabriel Sebastian; Niederle, María Virginia; Minahk, Carlos Javier; Picariello, Gianluca; Nader, Maria Elena Fatima; et al.; Nisin Z produced by lactococcus lactis from bullfrog hatchery is active against litrobacter freundii, a red-leg syndrome related pathogen; Springer; World Journal of Microbiology; 33; 10; 10-2017; 186-198
Compartir
Altmétricas
 
Estadísticas
Visualizaciones: 82
Descargas: 5

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • Twitter
  • Instagram
  • YouTube
  • Sound Cloud

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

Ministerio
https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES