Artículo
Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons
Perez, Maria Florencia
; Contreras, Luciana
; Garnica, Nydia Merecedes; Fernandez Zenoff, Maria Veronica
; Farias, Maria Eugenia
; Sepulveda, Milena; Ramallo, Jaqueline; Dib, Julian Rafael
Fecha de publicación:
10/2016
Editorial:
Public Library of Science
Revista:
Plos One
ISSN:
1932-6203
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Economic losses caused by postharvest diseases represent one of the main problems of the citrus industry worldwide. The major diseases affecting citrus are the "green mold" and "blue mold", caused by Penicillium digitatum and P. italicum, respectively. To control them, synthetic fungicides are the most commonly used method. However, often the emergence of resistant strains occurs and their use is becoming more restricted because of toxic effects and environmental pollution they generate, combined with trade barriers to international markets. The aim of this work was to isolate indigenous killer yeasts with antagonistic activity against fungal postharvest diseases in lemons, and to determine their control efficiency in in vitro and in vivo assays. Among 437 yeast isolates, 8.5% show to have a killer phenotype. According to molecular identification, based on the 26S rDNA D1/D2 domain sequences analysis, strains were identified belonging to the genera Saccharomyces, Wickerhamomyces, Kazachstania, Pichia, Candida and Clavispora. Killers were challenged with pathogenic molds and strains that caused the maximum in vitro inhibition of P. digitatum were selected for in vivo assays. Two strains of Pichia and one strain of Wickerhamomyces depicted a significant protection (p <0.05) from decay by P. digitatum in assays using wounded lemons. Thus, the native killer yeasts studied in this work showed to be an effective alternative for the biocontrol of postharvest fungal infections of lemons and could be promising agents for the development of commercial products for the biological control industry.
Palabras clave:
Killer Yeast
,
Lemon
,
Biological Control
,
Penicillium
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Perez, Maria Florencia; Contreras, Luciana; Garnica, Nydia Merecedes; Fernandez Zenoff, Maria Veronica; Farias, Maria Eugenia; et al.; Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons; Public Library of Science; Plos One; 11; 10; 10-2016; 1-21; e0165590
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