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dc.contributor.author Perez, Maria Florencia
dc.contributor.author Contreras, Luciana
dc.contributor.author Garnica, Nydia Merecedes
dc.contributor.author Fernandez Zenoff, Maria Veronica
dc.contributor.author Farias, Maria Eugenia
dc.contributor.author Sepulveda, Milena
dc.contributor.author Ramallo, Jaqueline
dc.contributor.author Dib, Julian Rafael
dc.date.available 2018-04-04T15:21:55Z
dc.date.issued 2016-10
dc.identifier.citation Perez, Maria Florencia; Contreras, Luciana; Garnica, Nydia Merecedes; Fernandez Zenoff, Maria Veronica; Farias, Maria Eugenia; et al.; Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons; Public Library of Science; Plos One; 11; 10; 10-2016; 1-21; e0165590
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/11336/40676
dc.description.abstract Economic losses caused by postharvest diseases represent one of the main problems of the citrus industry worldwide. The major diseases affecting citrus are the "green mold" and "blue mold", caused by Penicillium digitatum and P. italicum, respectively. To control them, synthetic fungicides are the most commonly used method. However, often the emergence of resistant strains occurs and their use is becoming more restricted because of toxic effects and environmental pollution they generate, combined with trade barriers to international markets. The aim of this work was to isolate indigenous killer yeasts with antagonistic activity against fungal postharvest diseases in lemons, and to determine their control efficiency in in vitro and in vivo assays. Among 437 yeast isolates, 8.5% show to have a killer phenotype. According to molecular identification, based on the 26S rDNA D1/D2 domain sequences analysis, strains were identified belonging to the genera Saccharomyces, Wickerhamomyces, Kazachstania, Pichia, Candida and Clavispora. Killers were challenged with pathogenic molds and strains that caused the maximum in vitro inhibition of P. digitatum were selected for in vivo assays. Two strains of Pichia and one strain of Wickerhamomyces depicted a significant protection (p <0.05) from decay by P. digitatum in assays using wounded lemons. Thus, the native killer yeasts studied in this work showed to be an effective alternative for the biocontrol of postharvest fungal infections of lemons and could be promising agents for the development of commercial products for the biological control industry.
dc.format application/pdf
dc.language.iso eng
dc.publisher Public Library of Science
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject KILLER YEAST
dc.subject LEMON
dc.subject BIOLOGICAL CONTROL
dc.subject PENICILLIUM
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-03-02T15:00:46Z
dc.journal.volume 11
dc.journal.number 10
dc.journal.pagination 1-21; e0165590
dc.journal.pais Estados Unidos
dc.journal.ciudad San Francisco
dc.description.fil Fil: Perez, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
dc.description.fil Fil: Contreras, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil Fil: Garnica, Nydia Merecedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil Fil: Fernandez Zenoff, Maria Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
dc.description.fil Fil: Sepulveda, Milena. S.a. San Miguel - Citricola; Argentina
dc.description.fil Fil: Ramallo, Jaqueline. S.a. San Miguel - Citricola; Argentina
dc.description.fil Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
dc.journal.title Plos One
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165590
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0165590
dc.conicet.fuente individual


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)