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Artículo

Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis

Soto Vargas, Verónica CarolinaIcon ; Fernández, María de Los ÁngelesIcon ; Galmarini, Claudio RomuloIcon ; Silva, María FernandaIcon
Fecha de publicación: 16/07/2014
Editorial: Royal Society of Chemistry
Revista: Analytical Methods
ISSN: 1759-9660
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
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Resumen

Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L-1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production.
Palabras clave: Onion Nectar , Spe , Capillary Electrophoresis , Phenolic Compounds
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/38965
DOI: http://dx.doi.org/10.1039/c4ay00240g
URL: http://pubs.rsc.org/en/Content/ArticleLanding/2014/AY/C4AY00240G#!divAbstract
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Articulos(CCT - MENDOZA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MENDOZA
Citación
Soto Vargas, Verónica Carolina; Fernández, María de Los Ángeles; Galmarini, Claudio Romulo; Silva, María Fernanda; Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis; Royal Society of Chemistry; Analytical Methods; 6; 13; 16-7-2014; 4878-4884
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