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Artículo

Polisaccharides influence on the interaction between tannin acid and haze active proteins in beer

Lataza Rovaletti, Mercedes; Benitez, Elisa InesIcon ; Martinez Amezaga, Nancy María JimenaIcon ; Peruchena, Nelida MariaIcon ; Sosa, Gladis Laura; Lozano, Jorge EnriqueIcon
Fecha de publicación: 08/2014
Editorial: Elsevier Science
Revista: Food Research International
ISSN: 0963-9969
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ingeniería Química

Resumen

Colloidal instability in beer is mainly caused by interactions between proteins and polyphenols. These two combine producing a visible haze that reduces the physical shelf life of beer. The haze active proteins (HAPs) react with tannic acid (TA); therefore, this reaction provides a way to determine HAP concentrations in beer. Beers also contain a number of constituents that may influence the protein-polyphenol haze formation. We used a response surface methodology to predict the influence of total polysaccharides (TPS) and proteins on beer haze. Experiments were carried out using the Central Composite Design (CCD) methodology. Samples of beer were prepared with variable concentrations of TPS and proteins. TPS concentrations ranged between 1.34 and 2.23gL-1 and proteins concentrations between 0.11 and 0.18gL-1. Results show that the increase in turbidity in response to TPS addition was similar to that in response to protein addition. Our regression analyses indicate a significant dependency and correlation between the observed values and the predicted response values (R2=97.87% and R2[adj]=95.75%). Furthermore, these values indicate that our experimental model can explain 95.75% of the total variation. Therefore, using TA as an indicator of the interaction of TPS with proteins, as commonly done, can lead to considerable errors, since the polysaccharides also react with TA, and this reaction actually causes a considerable increase in turbidity.
Palabras clave: Beer , Haze Active Proteins , Ionic Strength , Polysaccharides , Tannic Acid
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/37158
URL: http://www.sciencedirect.com/science/article/pii/S0963996914001756
DOI: http://dx.doi.org/10.1016/j.foodres.2014.03.017
Colecciones
Articulos(CCT - NORDESTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Articulos(PLAPIQUI)
Articulos de PLANTA PILOTO DE INGENIERIA QUIMICA (I)
Citación
Lataza Rovaletti, Mercedes; Benitez, Elisa Ines; Martinez Amezaga, Nancy María Jimena; Peruchena, Nelida Maria; Sosa, Gladis Laura; et al.; Polisaccharides influence on the interaction between tannin acid and haze active proteins in beer; Elsevier Science; Food Research International; 62; 8-2014; 779-785
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