Artículo
Phenolic characterization and antimicrobial activity of folk medicinal plant extracts for their applications in olive production
Boiteux, Joana Jaqueline
; Soto Vargas, Verónica Carolina
; Pizzuolo, Pablo Humberto; Lucero, Gabriela; Silva, María Fernanda
Fecha de publicación:
03/2014
Editorial:
Wiley VCH Verlag
Revista:
Electrophoresis
ISSN:
0173-0835
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Phytophthora spp is important in plant pathology due to the importance of the diseases it causes. In olive trees, severe damages are caused by the disease known as "dry branch" occasioned by Phytophthora nicotianae, P. citrophthora and P. palmivora. Much effort has been made to find efficient methods of control, with a low negative impact on environment. In this regard, treatment with plant extracts is a valid strategy. The aims of the present study are (i) to determine the polyphenol composition of extracts of Thymus vulgaris, Origanum vulgare, Matricaria recutita, and Larrea divaricata by CZE, (ii) correlate the analytical composition of these extracts with the inhibition on the mycelial growth, and (iii) determine the individual antimicrobial activity of the most active ingredients. A simple methodology was developed for the determination of catechin, naringenin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in plant extracts by CZE. The extraction of phenolic compounds in extract was performed by a miniaturized solid phase extraction using a home-made minicolumn packed with suitable filtering material (C18, 50 mg). The optimized analyses conditions were: 30 mM boric acid buffer, pH 9.50; capillary, 57 cm full length, 50 cm effective length, 75 μm id, hydrodynamic injection 30 mbar, 2 s; 25 kV; 25°C, detection by UV absorbance at 290 nm. Sample results suggest that phenolic composition seems to have a great influence on inhibition of pathogens. The highest inhibitions of mycelial growth were observed for cinnamic acid and naringenin. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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Articulos(IBAM)
Articulos de INST.DE BIOLOGIA AGRICOLA DE MENDOZA
Articulos de INST.DE BIOLOGIA AGRICOLA DE MENDOZA
Citación
Boiteux, Joana Jaqueline; Soto Vargas, Verónica Carolina; Pizzuolo, Pablo Humberto; Lucero, Gabriela; Silva, María Fernanda; Phenolic characterization and antimicrobial activity of folk medicinal plant extracts for their applications in olive production; Wiley VCH Verlag; Electrophoresis; 35; 11; 3-2014; 1709-1718
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