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Artículo

28-mer fragment derived from Enterocin CRL35 displays an unexpected bactericidal effect on listeria cells

Masias, Ruth EmilseIcon ; da Silva Sanches, Paulo Ricardo; Dupuy, Fernando GabrielIcon ; Acuña, Leonardo; Bellomio, AugustoIcon ; Cilli, Eduardo; Saavedra, Maria LucilaIcon ; Minahk, Carlos JavierIcon
Fecha de publicación: 05/2015
Editorial: Bentham Science Publishers
Revista: Protein and Peptide Letters
ISSN: 0929-8665
e-ISSN: 1875-5305
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Two shorter peptides derived from enterocin CRL35, a 43-mer bacteriocin, were synthesized i.e. the N-terminal fragment spanning from residues 1 to 15, and a 28-mer fragment that represents the C-terminal of enterocin CRL35, the residues 16 to 43. The separate peptides showed no activity when combined. On one hand, the 28-mer peptide displayed an unpredicted antimicrobial activity. On the other, 15- mer peptide had no consistent anti-Listeria effect. The dissociation constants calculated from experimental data indicated that all peptides could bind at similar extent to the sensitive cells. However, transmembrane electrical potential was not dissipated to the same level by the different peptides; whereas the full-length and the C-terminal 28-mer fragment induced almost full dissipation, 15-mer fragment produced only a slow and incomplete effect. Furthermore, a different interaction of each peptide with membranes was demonstrated based on studies carried out with liposomes, which led us to conclude that activity was related to structure rather than to net positive charges. These results open up the possibility of designing new peptides based on the 28-mer fragment with enhanced activity, which would represent a promising approach for combating Listeria and other pathogens.
Palabras clave: Bacteriocins , Enterocin Crl35 , Listeria , Synthetic Peptides
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/28427
DOI: http://dx.doi.org/10.2174/0929866522666150506094300
URL: http://www.eurekaselect.com/131041/article
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Articulos(CERELA)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Citación
Masias, Ruth Emilse; da Silva Sanches, Paulo Ricardo; Dupuy, Fernando Gabriel; Acuña, Leonardo; Bellomio, Augusto; et al.; 28-mer fragment derived from Enterocin CRL35 displays an unexpected bactericidal effect on listeria cells; Bentham Science Publishers; Protein and Peptide Letters; 22; 6; 5-2015; 482-488
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