Artículo
Magnetic amyloid-based biocatalyst for the hydrolysis of urea
de Athayde Moncorvo Collado, Alejandro
; Socias, Sergio Benjamin
; González Lizárraga, Florencia; Ploper, Diego
; Vera Pingitore, Esteban
; Chehin, Rosana Nieves
; Chaves, Analia Silvina






Fecha de publicación:
02/2024
Editorial:
Elsevier
Revista:
Food Chemistry
ISSN:
0308-8146
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The presence of urea in wines and other alcoholic beverages represents a critical problem since it can chemically react with ethanol, which leads to the formation of ethyl carbamate, a carcinogenic agent according to the World Health Organization. Here we report the creation of a biocatalyst for the hydrolysis of urea, which could potentially be used before bottling alcoholic drinks. For this, the effective surface area of streptavidin-labeled magnetic microparticles was amplified by functionalization with biotin-labeled hen egg lysozyme amyloid fibers. Subsequently, by using copper and hydrogen peroxide induced cross-linking of unmodified proteins (CHICUP), soybean urease was immobilized to the fibers. This gave rise to a magnetic biocatalyst with remarkable urease activity, which was maintained even after 10 reuses. We propose that this strategy could be used as a platform for immobilizing other molecules to design and develop a myriad of biocatalysts for the food industry.
Palabras clave:
AMYLOID FIBRIL
,
ETHYL CARBAMATE
,
IMMOBILIZATION
,
MAGNETIC PARTICLE
,
UREASE
,
WINE
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Articulos (IMMCA)
Articulos de INSTITUTO DE INVESTIGACIONES EN MEDICINA MOLECULAR Y CELULAR APLICADA DEL BICENTENARIO
Articulos de INSTITUTO DE INVESTIGACIONES EN MEDICINA MOLECULAR Y CELULAR APLICADA DEL BICENTENARIO
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
de Athayde Moncorvo Collado, Alejandro; Socias, Sergio Benjamin; González Lizárraga, Florencia; Ploper, Diego; Vera Pingitore, Esteban; et al.; Magnetic amyloid-based biocatalyst for the hydrolysis of urea; Elsevier; Food Chemistry; 433; 2-2024; 1-9
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