Artículo
Fungal Laccases and Fumonisin Decontamination in Co-Products of Bioethanol from Maize
Bossa, Marianela
; Monesterolo, Noelia Edith
; Monge, Maria del Pilar
; Rhein, Paloma; Chulze, Sofia Noemi
; Alaniz Zanon, Maria Silvina
; Chiotta, María Laura
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Fecha de publicación:
10/08/2024
Editorial:
Multidisciplinary Digital Publishing Institute
Revista:
Toxins
e-ISSN:
2072-6651
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Maize (Zea mays L.) may be infected by Fusarium verticillioides and F. proliferatum, andconsequently contaminated with fumonisins (FBs), as well as the co-products of bioethanol intended for animal feed. Laccase enzymes have a wide industrial application such as mycotoxin degradation. The aims were to isolate and identify fungal laccase-producing strains, to evaluate laccase production, to determine the enzymatic stability under fermentation conditions, and to analyse the effectiveness in vitro of enzymatic extracts (EEs) containing laccases in degrading FB1. Strains belonging to Funalia trogii, Phellinus tuberculosus, Pleurotus ostreatus, Pycnoporus sanguineus and Trametes gallica species showed laccase activity. Different isoforms of laccases were detected depending on the evaluated species. For the FB1 decontamination assays, four enzymatic activities (5, 10, 15 and 20 U/mL) were tested, in the absence and presence of vanillic acid (VA) and 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) as redox mediators (1 and 10 mM). Trametes gallica B4-IMICO-RC EE was the most effective strain in buffer, achieving a 60% of FB1 reduction. Laccases included in EEs remained stable at different alcoholic degrees in maize steep liquor (MSL), but no significant FB1 reduction was observed under the conditions evaluated using MSL. This study demonstrate that although laccases could be good candidates for the development of a strategy to reduce FB1, further studies are necessary to optimise this process in MSL.
Palabras clave:
FUNGAL LACCASES
,
FUMONISIN B1
,
MAIZE STEEP LIQUOR
,
DECONTAMINATION
,
FOOD SAFETY
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Identificadores
Colecciones
Articulos (IMICO)
Articulos de INSTITUTO DE INVESTIGACION EN MICOLOGIA Y MICOTOXICOLOGIA
Articulos de INSTITUTO DE INVESTIGACION EN MICOLOGIA Y MICOTOXICOLOGIA
Articulos (INBIAS)
Articulos de INSTITUTO DE BIOTECNOLOGIA AMBIENTAL Y SALUD
Articulos de INSTITUTO DE BIOTECNOLOGIA AMBIENTAL Y SALUD
Citación
Bossa, Marianela; Monesterolo, Noelia Edith; Monge, Maria del Pilar; Rhein, Paloma; Chulze, Sofia Noemi; et al.; Fungal Laccases and Fumonisin Decontamination in Co-Products of Bioethanol from Maize; Multidisciplinary Digital Publishing Institute; Toxins; 16; 8; 10-8-2024; 1-18
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