Artículo
Interaction of tannase from Aspergillus niger with polycations applied to its primary recovery
Rodriguez Durán, Luis V.; Spelzini, Darío
; Boeris, Valeria
; Aguilar, Cristóbal N.; Picó, Guillermo Alfredo
Fecha de publicación:
07/2012
Editorial:
Elsevier Science
Revista:
Colloids and Surfaces B: Biointerfaces
ISSN:
0927-7765
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Tannase from Aspergillus niger was partitioned in aqueous two-phase systems composed by polyethyleneglycol of molar mass 400, 600 and 1000 and potassium phosphate. Tannase was found to be partitioned toward the salt-rich phase in all systems, with partition coefficients lower than 0.5. Partition coefficients values and low entropic and enthalpic changes associated with tannase partition suggest that the entropic effect may be the driving force of the concentration of the enzyme in the bottom phase due to the high molar mass of the enzyme. The process was significantly influenced by the top phase/bottom phase volume ratio. When the fungal culture broth was partitioned in these systems, a good performance was found, since the enzyme recovery in the bottom phase of the system composed by polyethyleneglycol 1000 was around 96% with a 7.0-fold increase in purity.
Palabras clave:
Tannasse
,
Aquous Two Phases
,
Bioseparation
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Identificadores
Colecciones
Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Citación
Rodriguez Durán, Luis V.; Spelzini, Darío; Boeris, Valeria; Aguilar, Cristóbal N.; Picó, Guillermo Alfredo; Interaction of tannase from Aspergillus niger with polycations applied to its primary recovery; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 101; 7-2012; 392-397
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