An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine

Abstract

A biosensor based on the immobilization of Lactate oxidase in laponite - organosilasesquioxane films on glassy carbon electrode for the quantification of l-lactate in wine and dairy products is presented. The bioelectrode showed a very high sensitivity (0.33 ± 0.01) A cm-2 M-1 and a short time response (10 s) for less than 1 U of enzyme. No significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our l-lactate determinations were in good agreement with those of two standard methods.