Artículo
Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
Pujato, Silvina
; Galliani, Valentina; Irazoqui, José Matías
; Amadío, Ariel Alberto; Quiberoni, Andrea del Lujan
; Mercanti, Diego Javier
Fecha de publicación:
07/2021
Editorial:
Elsevier
Revista:
International Dairy Journal
ISSN:
0958-6946
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections.
Palabras clave:
CRISPR
,
BACTERIAS ÁCIDO LÁCTICAS
,
FAGOS
,
MECANISMO DE FAGORRESISTENCIA
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos (IDICAL)
Articulos de INSTITUTO DE INVESTIGACION DE LA CADENA LACTEA
Articulos de INSTITUTO DE INVESTIGACION DE LA CADENA LACTEA
Articulos(INLAIN)
Articulos de INST.DE LACTOLOGIA INDUSTRIAL
Articulos de INST.DE LACTOLOGIA INDUSTRIAL
Citación
Pujato, Silvina; Galliani, Valentina; Irazoqui, José Matías; Amadío, Ariel Alberto; Quiberoni, Andrea del Lujan; et al.; Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus; Elsevier; International Dairy Journal; 118; 7-2021; 1-11
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