Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Diversity of volatile organic compound production from leucine and citrate in Enterococcus faecium

D'angelo, Matilde ElviraIcon ; Martino, Gabriela PaulaIcon ; Blancato, Victor SebastianIcon ; Espariz, MartinIcon ; Hartke, Axel; Sauvageot, Nicolas; Benachour, Abdellah; Alarcon, Sergio HugoIcon ; Magni, ChristianIcon
Fecha de publicación: 12/2019
Editorial: Springer
Revista: Applied Microbiology and Biotechnology
ISSN: 0175-7598
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Enterococcus faecium is frequently isolated from fermented food; in particular, they positively contribute to the aroma compounds generation in traditional cheese. Citrate fermentation is a desirable property in these bacteria, but this feature is not uniformly distributed among E. faecium strains. In the present study three selected E. faecium strains, IQ110 (cit-), GM70 (cit+ type I) and Com12 (cit+ type II) were analyzed in their production of aroma compounds in milk. End products and volatile organic compounds (VOCs) were determined by solid phase micro-extraction combined with gas chromatography-mass spectrometry (SPME GC-MS). Principal component analysis (PCA) of aroma compounds profiles revealed a different VOCs composition for the three strains. In addition, resting cells experiments of E. faecium performed in the presence of leucine, citrate or pyruvate aroma compound precursors, allowed us to determine metabolic differences between the studied strains. GM70 (cit+ type I) showed an active citrate metabolism, with increased levels of diacetyl and acetoin generation relatively to Com12 or to citrate defective IQ110 strains. In addition, in our experimental conditions, we found a defective citrate fermenting phenotype for the Com12 strain, while its leucine degradation and pyruvate metabolism are conserved. In conclusion, rational selection of E. faecium strains could be performed based on genotypic and phenotypic analyzes. This would result in a performing strain, such as GM70, that could positively contribute to flavor, with typical notes of diacetyl, acetoin, 3-methyl butanal and 3-methyl butanol in an adjuvant culture.
Palabras clave: ENTEROCOCCUS FAECIUM , AROMA COMPOUND , LEUCINE , CITRATE
Ver el registro completo
 
Archivos asociados
Tamaño: 1.189Mb
Formato: PDF
.
Solicitar
Licencia
info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/160853
URL: http://link.springer.com/10.1007/s00253-019-10277-4
DOI: http://dx.doi.org/10.1007/s00253-019-10277-4
Colecciones
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
D'angelo, Matilde Elvira; Martino, Gabriela Paula; Blancato, Victor Sebastian; Espariz, Martin; Hartke, Axel; et al.; Diversity of volatile organic compound production from leucine and citrate in Enterococcus faecium; Springer; Applied Microbiology and Biotechnology; 104; 12-2019; 1175-1186
Compartir
Altmétricas
 

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • X Conicet Digital
  • Instagram
  • YouTube
  • Sound Cloud
  • LinkedIn

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES