Artículo
A novel approach to ameliorate experimental milk allergy based on the oral administration of a short soy cross-reactive peptide
Candreva, Ángela María
; Smaldini, Paola Lorena
; Cauerhff, Ana; Petruccelli, Silvana
; Docena, Guillermo H.
Fecha de publicación:
12/2020
Editorial:
Elsevier
Revista:
Food Chemistry
ISSN:
0308-8146
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Food allergy is on the rise, and preventive/therapeutic procedures are needed. We explored a preventive protocolfor milk allergy with the oral administration of a Gly-m-Bd-30K soy-derived peptide that contains cross-reactiveepitopes with bovine caseins. B/T-cross-reactive epitopes were mapped using milk-specific human sera andmonoclonal antibodies on overlapping and recombinant peptides of Gly-m-Bd-30K by SPOT and cell proliferationassays. Bioinformatics tools were used to characterize epitopes on the 3D-modelled molecule, and to predict thebinding to HLA alleles. The peptide was orally administrated to mice that were then IgE-sensitized to milkproteins. Immunodominant B-epitopes were mainly located on the surface of the Nt-fragment. The use of a soypeptide-containing an immunodominant cross-reactive T-epitope, along with a single B epitope, prevents IgEmediatedmilk sensitization through the induction of Th1-mediated immunity and induction of blocking IgG.The use of a safe soy-peptide may represent a promising alternative for preventing milk allergy.
Palabras clave:
CROSS-REACTIVITY
,
FOOD ALLERGY
,
IGE
,
MILK ALLERGY
,
PREVENTION
,
SOY PEPTIDE
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Articulos(IIFP)
Articulos de INST. DE ESTUDIOS INMUNOLOGICOS Y FISIOPATOLOGICOS
Articulos de INST. DE ESTUDIOS INMUNOLOGICOS Y FISIOPATOLOGICOS
Citación
Candreva, Ángela María; Smaldini, Paola Lorena; Cauerhff, Ana; Petruccelli, Silvana; Docena, Guillermo H.; A novel approach to ameliorate experimental milk allergy based on the oral administration of a short soy cross-reactive peptide; Elsevier; Food Chemistry; 346; 128926; 12-2020; 1-11
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