Artículo
Microcin J25 inhibits ubiquinol oxidase activity of purified cytochrome bd-I from Escherichia coli
Galván, Adriana Emilce
; Chalon, Miriam Carolina
; Ríos Colombo, Natalia Soledad
; Schurig Briccio, Lici Ariane
; Sosa Padilla Araujo, Bernardo
; Gennis, Robert B.; Bellomio, Augusto
Fecha de publicación:
05/2019
Editorial:
Elsevier France-editions Scientifiques Medicales Elsevier
Revista:
Biochimie
ISSN:
0300-9084
e-ISSN:
1638-6183
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Microcin J25 (MccJ25), an antimicrobial peptide, targets the respiratory chain but the exact mechanism by which it does so remains unclear. Here, we reveal that MccJ25 is able to inhibit the enzymatic activity of the isolated cytochrome bd-I from E. coli and induces at the same time production of reactive oxygen species. MccJ25 behaves as a dose-dependent weak inhibitor. Intriguingly, MccJ25 is capable of producing a change in the oxidation state of cytochrome bd-I causing its partial reduction in the presence of cyanide. These effects are specific for cytochrome bd-I, since the peptide is not able to act on purified cytochrome bo 3 .
Palabras clave:
CYTOCHROME BD-I
,
REDOX PEPTIDE
,
RESPIRATORY CHAIN
,
ROS
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Galván, Adriana Emilce; Chalon, Miriam Carolina; Ríos Colombo, Natalia Soledad; Schurig Briccio, Lici Ariane; Sosa Padilla Araujo, Bernardo; et al.; Microcin J25 inhibits ubiquinol oxidase activity of purified cytochrome bd-I from Escherichia coli; Elsevier France-editions Scientifiques Medicales Elsevier; Biochimie; 160; 5-2019; 141-147
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