Artículo
Detection and identification of Lactobacillus helveticus bacteriophages by PCR
Fecha de publicación:
12/2008
Editorial:
Cambridge University Press
Revista:
Journal of Dairy Research
ISSN:
0022-0299
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A PCR protocol for detection of Lactobacillus helveticus bacteriophages was optimized. PCR was designed taking into account the sequence of the lys gene of temperate bacteriophage W-0303 and optimized to obtain a fragment of 222 bp using different Lb. helveticus phages from our collection. PCR was applied to total phage DNA extracted from 53 natural whey starters used for the production of Grana cheese and all gave the expected fragment. The presence of actively growing phages in the cultures was verified by traditional tests. Several PCR products of the lys gene were sequenced and aligned. The resulting sequences showed variable heterogeneity between the phages.
Palabras clave:
LACTOBACILLUS HELVETICUS
,
BACTERIOPHAGES
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INLAIN)
Articulos de INST.DE LACTOLOGIA INDUSTRIAL
Articulos de INST.DE LACTOLOGIA INDUSTRIAL
Citación
Zago, Miriam; Rossetti, Lia; Reinheimer, Jorge Alberto; Carminati, Domenico; Giraffa, Giorgio; Detection and identification of Lactobacillus helveticus bacteriophages by PCR; Cambridge University Press; Journal of Dairy Research; 75; 2; 12-2008; 196-201
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