Artículo
Genistein inhibits contractile force, intracellular Ca2+ increase and Ca2+ oscillations induced by serotonin in rat aortic smooth muscle
Speroni Aguirre, Francisco José
; Sierra Rebolledo, Alejandro; Añon, Maria Cristina
; Tanzi, Federico; Milesi, Verónica
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Fecha de publicación:
06/2007
Editorial:
Servicio Publicaciones Universidad Navarra
Revista:
Journal of Physiology and Biochemistry
ISSN:
1138-7548
e-ISSN:
1877-8755
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The soy-derived isoflavones genistein and daidzein affect the contractile state of different kinds of smooth muscle. We describe acute effects of genistein and daidzein on contractile force and intracellular Ca2+ concentration ([Ca2+]i) in in situ smooth muscle of rat aorta. Serotonin (5-HT) (2 μM) or a depolarizing high K+ solution produced the contraction of aortic rings, which were immediately relaxed by 20 μM genistein and by 20 μM daidzein. Accordingly, both 5-HT and a high K+ solution increased the [Ca2+]i in in situ smooth muscle cells. Genistein strongly inhibited the [Ca2+]i increase evoked by 5-HT (74.0±7.3%, n=11, p<0.05), and had a smaller effect on high K+ induced [Ca2+]i increase (19.9±4.0%, n=7, p<0.05). The K+ channels blocker tetraethylammonium (TEA) (0.5 mM) diminished genistein effects on 5-HT-induced [Ca2+]i increase. Interestingly, during prolonged application of 5-HT, the [Ca2+]i oscillated and a short (90 s) preincubation with genistein (20 μM) significantly diminished the frequency of the oscillations. This effect was totally abolished by TEA. In conclusion, in rat aortic smooth muscle, genistein is capable of diminishing the increase in [Ca2+]i and in force evoked by 5-HT and high K+ solution, and of decreasing the frequency of [Ca2+]i oscillations induced by 5-HT. The short time required by genistein, and the relaxing effect of daidzein suggest that tyrosine kinases inhibition is not involved. The small inhibiting effect of genistein on the [Ca2+]i increase evoked by high K+ and the effect of TEA point to the activation by genistein of calcium-activated K+ channels.
Palabras clave:
Genisteina
,
isoflavonas
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CENEXA)
Articulos de CENTRO DE ENDOCRINOLOGIA EXP.Y APLICADA (I)
Articulos de CENTRO DE ENDOCRINOLOGIA EXP.Y APLICADA (I)
Articulos(CIDCA)
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Citación
Speroni Aguirre, Francisco José; Sierra Rebolledo, Alejandro; Añon, Maria Cristina; Tanzi, Federico; Milesi, Verónica; Genistein inhibits contractile force, intracellular Ca2+ increase and Ca2+ oscillations induced by serotonin in rat aortic smooth muscle; Servicio Publicaciones Universidad Navarra; Journal of Physiology and Biochemistry; 63; 2; 6-2007; 143-151
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