Estudios citogenéticos en cuatro taxones de Canna (Cannaceae) de la provincia de Buenos Aires, Argentina
; Ciciarelli, María de Las Mercedes ; Rolleri, Cristina Hilda 
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10/2017
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Universidad Complutense de Madrid
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Botanica Complutensis
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0214-4565
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Se investigaron los números cromosómicos y los cariotipos de tres especies y un híbrido del género Canna, que crecen en la Argentina y también se encuentran distribuidos en el neotrópico. Mediante una tinción cromosómica convencional se analizaron C. indica, C. fuchsina, C. coccinea y el híbrido C. x generalis. Todos los taxones estudiados resultaron diploides (2n = 18). Las tres especies presentaron cariotipos similares simétricos, con cromosomas pequeños, mayormente metacéntricos aunque con algunos pares cromosómicos submetacéntricos. En C. coccinea y C. fuchsina, y por primera vez en el género, se aplicó la técnica de bandeo fluorescente DAPI sobre interfases y metafases mitóticas, con el objetivo de detectar regiones de heterocromatina constitutiva rica en Adenina-Timina (A-T). Los resultados obtenidos permitieron el reconocimiento de pares cromosómicos y demostraron la utilidad de este método para detectar la variación interespecífica en la distribución cromosómica de la heterocromatina rica en A-T en Canna. Se sugiere que el material estudiado de C. x generalis es un diploide en el que el 2n = 18 no contradice la hipótesis sobre su origen, ya que el número hallado coincide con el de uno de los progenitores, C. indica, y con el que se conoce para otras especies parentales.
Chromosome numbers and karyotypes of three species and a hybrid of the genus Canna growing in Argentina, and also distributed in the Neotrópico, were investigated here. Chromosome counts were performed in C. indica, C. fuchsina, C. coccinea and the hybrid C. x generalis with the conventional chromosomal stain techniques. All studied taxa proved to be diploid (2n = 18). Three species showed similar symmetric karyotypes, with mainly metacentric small and short chromosomes, though some submetacentric chromosome pairs were observed. Fluorescent DAPI chromosome banding technique was applied on interphases and mitotic metaphases of C. coccinea and C. fuchsina, this procedure for the first time in the genus, with the aim to detect the presence of regions of constitutive heterochromatin rich in Adenine-Thymine (A-T). The results obtained allowed the recognition of chromosomal pairs and demonstrated the usefulness of this method to detecting the interspecific variation in the chromosomal distribution of A-T rich heterochromatin in Canna. The diploid number 2n=18 found in the studied material of C. x generalis does not contradicts the hypothesis on its origin, since this chromosome count coincide with the present in one of the progenitors, C. indica, and is also known for other parental species.
Chromosome numbers and karyotypes of three species and a hybrid of the genus Canna growing in Argentina, and also distributed in the Neotrópico, were investigated here. Chromosome counts were performed in C. indica, C. fuchsina, C. coccinea and the hybrid C. x generalis with the conventional chromosomal stain techniques. All studied taxa proved to be diploid (2n = 18). Three species showed similar symmetric karyotypes, with mainly metacentric small and short chromosomes, though some submetacentric chromosome pairs were observed. Fluorescent DAPI chromosome banding technique was applied on interphases and mitotic metaphases of C. coccinea and C. fuchsina, this procedure for the first time in the genus, with the aim to detect the presence of regions of constitutive heterochromatin rich in Adenine-Thymine (A-T). The results obtained allowed the recognition of chromosomal pairs and demonstrated the usefulness of this method to detecting the interspecific variation in the chromosomal distribution of A-T rich heterochromatin in Canna. The diploid number 2n=18 found in the studied material of C. x generalis does not contradicts the hypothesis on its origin, since this chromosome count coincide with the present in one of the progenitors, C. indica, and is also known for other parental species.
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