Detection of human DNA in larvae and adults secretions of Calliphora vicina (Diptera: Calliphoridae) fed on human liver

Abstract

A través del análisis de ADN extraído del contenido intestinal de larvas que consumen tejido humano, surge una metodología para determinar la fuente de alimento. En esta investigación, se proporcionó hígado humano como alimento a larvas y adultos de Calliphora vicina Robineau-Desvoidy (Diptera: Calliphoridae). El énfasis se centró principalmente en evaluar la calidad y persistencia del material genético humano presente en el intestino de las larvas, así como en las secreciones de los adultos que emergieron de larvas alimentadas con hígado humano. Los hallazgos revelaron que solo dentro del intestino de las larvas se puede discernir material genético humano viable, proveniente de la fuente de alimento, incluso después de un período de ayuno de dos horas.Through the analysis of DNA extracted from the gut contents of larvae known to consume human tissue, a methodology emerges for determining the source of their sustenance. In this study, human liver was provided as a food source to larvae and adults of Calliphora vicina Robineau-Desvoidy (Diptera: Calliphoridae). Emphasis was primarily placed on evaluating the quality and persistence of human genetic material present in the gut of larvae as well as in the secretions of adults that emerged from larvae fed on human liver. The findings revealed that viable genetic material appropriate for human identification could be discerned only within the larvae gut, even after a two-hour fasting period.