Aquaporin-9 is expressed in villous trophoblast mitochondria
; Szpilbarg, Natalia ; Damiano, Alicia Ermelinda Otros responsables
Fecha
2019
Editorial
Elsevier
Revista
Título del libro
Nombre del evento
International Federation of Placenta Association Meeting
Fecha del evento
2019-09-10
Institución organizadora
International Federation of Placenta Associations
Latin American Society for Maternal Fetal Interaction and Placenta
Latin American Society for Maternal Fetal Interaction and Placenta
ISSN
0143-4004
eISSN
ISBN
Idioma
Inglés
Tipo del evento
Reunión
Clasificación temática
Palabras clave
Resumen
OBJECTIVE: To explore the expression of AQP9 in the heavy/large and light/small mitochondrial subpopulations that coexist in the villous trophoblast of term placenta.MATERIAL AND METHODS: This study was approved by the ethics committee of Hospital Nacional Prof. Dr. A. Posadas. Human chorionic villi were fragmented, and tissue was shaken in HES buffer (10 mM HEPES-KOH, 0.1 mM EGTA, 250 mM sucrose), pH 7.4, with protease inhibitors. Differential centrifugation was performed to obtain mitochondrial, microsomal and cytosolic fractions. From mitochondrial fraction, heavy/large and light/small mitochondrial populations were also isolated by differential centrifugation. Localization was confirmed by immunofluorescence assay.
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