Beneficial activity of Enterococcus faecalis CECT7121 in the anti-lymphoma protective response

Abstract

Aims: To study the anti-tumour effects of Enterococcus faecalis CECT7121 on LBC cells, an aggressive murine T-cell lymphoma that kills the host in 18 days when is intraperitoneally (ip) administrated. Methods and Results: In vitro studies have shown that LBC cell proliferation was inhibited by Ent. faecalis CECT7121 stimulus in a dose dependent manner, inducing apoptosis. The production of ceramide was involved in the latter effect. In order to undertake in vivo studies, syngeneic BALB/c mice pre-treated ip with Ent. faecalis CECT7121 (2.5×10(8) CFU) were challenged ip with LBC cells (1.0×10(6) cells) the day after. On day 30 post-inoculation of LBC cells, 70% of Ent. faecalis CECT7121 pre-treated mice survived, whereas no survivals were recorded in the control group. A group of surviving mice was re-challenged with LBC cells and 89% of them survived. Upon stimulation with irradiated LBC cells, spleen cell proliferation, high IFNγ, IL-12 and IL-10 levels were observed in surviving animals. Conclusions: Ent. faecalis CECT7121 affected multiple factors of the tumour establishment by: down-regulating the LBC cell proliferation, and inducing apoptosis in these cells; and enhancing the immune response which protects animals from lymphoma challenge and rechallenge. Significance and Impact of the Study: This study demonstrate that Ent. faecalis CECT7121 has potential as a probiotic that could facilitate the development of novel complements to therapeutic strategies against oncological diseases.