Culturing bacteria from caenorhabditis elegans gut to assess colonization proficiency

Abstract

Determining an accurate count of intestinal bacteria from Caenorhabditis elegans is onecritical way to assess colonization proficiency by a given bacteria. This can be accomplished by culturingappropriate dilutions of worm gut bacteria on selective or differential agarized media. Because of thehigh concentration of bacteria in gut worm, dilution is necessary before plating onto growth media.Serial dilutions can reduce the concentration of the original intestinal sample to levels low enough forsingle colonies to be grown on media plates, allowing for the calculation of the initial counts of bacteriain the intestinal sample.