Shifts in rhizospheric bacterial communities of perennial ryegrass after organic and inorganic fertilization
; Villamil, Maria Bonita ; Zabaloy, Maria Celina Otros responsables
Fecha
Publicador:
Consejo Nacional de Investigaciones Científicas y Técnicas
Fecha de depósito:
24/04/2025
Fecha de creación:
06/2024
Clasificación temática
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Resumen
The dataset comes from a 16S rRNA amplicon sequencing experiment using metagenomic DNA from rhizosphere soil samples of Lolium perenne L. This study aimed to evaluate the impact of different fertilization treatments (anaerobic digestate, urea, or the combination of both) on bacterial community structure, alpha diversity, and predicted functional profiles. The study was conducted in the greenhouse under a complete randomized design.
Métodos
The study was conducted in the greenhouse under a complete randomized design with four fertilization treatments: unfertilized control, anaerobic digestate, inorganic fertilizer (urea), and combined fertilizer (urea + anaerobic digestate). Fertilizers were added at the same total dose (106 mg N-NH4+ g-1 soil, equal to 140 kg N-NH4+ ha-1). More details of the greenhouse experimental design can be found in Allegrini M, Iocoli GA, Zabaloy MC. Environ Sci Pollut Res. 2024;31(34):47132–47143. doi:10.1007/s11356-024-34279-6. DNA was extracted from soil samples using the commercial kit ADN Puriprep-Suelo kit (Inbio Highway, Tandil, Argentina). Sequencing was conducted at the DNA Service Laboratory, Roy J. Carver Biotechnology Center, University of Illinois (Urbana-Champaign, United States) using the primers V4_515F_New (5'-GTGYCAGCMGCCGCGGTAA-3') and V4_806R_New (5'-GGACTACNVGGGTWTCTAAT-3') on an Illumina MiSeq flowcell for 251 cycles from each end of the fragments (2x250 nt reads)
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