Chemokine and prolactin secretion is modified by Ang II and Ang-(1-7) in decidualized compared to non-decidualized human endometrial stromal cells (T-HESC)

Abstract

To analyze the effect of Ang II and Ang-(1-7) on the secretory profile of human endometrial stromal cells (T-HESC) decidualized in vitro. T-HESC were incubated or not (control) with dibutiryl-cAMP+medroxyprogesterone acetate (db-cAMP+MPA), with or without estradiol (E2) for 4 or 6 days (d). The peptides (125-500 nM) were added at time 0, or 4d. The participation of Ang II receptors, Ang-(1-7) receptor Mas, and NFAT, was analyzed by preincubation with losartan, PD123319, A-779, and INCA-6, respectively, prior to stimulation with the peptides. Prolactin (PRL), IL-8 and MCP-1 levels in 2 or 6d culture supernatants, were determined by ELISA. Differences were considered statistically significant with p <0.05. Ang II nor Ang-(1-7) induced PRL secretion. Respect to control, IL-8, but not MCP-1 levels, increased after 2d with Ang II, effect inhibited by losartan.Ang II or Ang-(1-7) cannot induce decidualization of T-HESC; once induced, it would be enhanced by Ang II and disadvantaged by Ang-(1-7). The peptides differentially regulates the production of chemokines in the endometrial stroma, showing opposite effects in non-decidualized compared to decidualized cells. Ang II and Ang-(1-7) would be acting through AT1R/NFAT and Mas, respectively.