Modified atmosphere affects glucosinolate metabolism during postharvest storage of broccoli

Abstract

Broccoli is a vegetable with a growing consumer demand worldwide due to its important nutritional properties, including highamounts of glucosinolates. However, this vegetable has a short postharvest life and quickly loses its organoleptic and nutritionalquality. Modified atmosphere is one of the numerous methodologies used to delay deterioration during postharvest storageof broccoli heads. In this study, the effect of a modified atmosphere during postharvest storage of broccoli on glucosinolatemetabolism was evaluated. Five glucosinolates were identified by using a UPLC system coupled to a mass spectrometer.We detected one aliphatic glucosinolate and four indolic glucosinolates, and their concentration was found to decrease duringstorage. The decrease in content was less marked, and in some cases, an increased level was observed in the treated samples.Moreover, the treatment made it possible to maintain higher expression (analyzed by real-time quantitative PCR) of geneslinked to glucosinolate biosynthesis. We also detected an increased expression of some genes related to indolic glucosinolatebiosynthesis. Overall, storage of broccoli in modified atmospheres allowed for maintaining better visual quality and higherlevels of glucosinolates.