Drug-Containing Layered Double Hydroxide/Alginate Dispersions for Tissue Engineering

Abstract

Alginate (Alg) is increasingly studied as a constitutive material of scaffolds for tissue engineering because of its easy gelation and biocompatibility, and the incorporation of drugs into its formulation allows for its functionality to be extended. However, Alg presents a low cell adhesion and proliferation capacity, and the incorporation of drugs may further reduce its biocompatibility. Layered double hydroxides (LDH) are promising fillers for Alg-based biomaterials, as they increase cell adhesion and interaction and provide drug storage and controlled release. In this work, LDH containing ibuprofen or naproxen were synthesized by coprecipitation at a constant pH and their properties upon their incorporation in Alg dispersions (LDH-Drug/Alg) were explored. Drug release profiles in simulated body fluid and the proliferation of pre-osteoblastic MC3T3-E1 cells by LDH-Drug/Alg dispersions were then evaluated, leading to results that confirm their potential as biomaterials for tissue engineering. They showed a controlled release with diffusive control, modulated by the in-situ formation of an Alg hydrogel in the presence of Ca2+ ions. Additionally, LDH-Drug/Alg dispersions mitigated the cytotoxic effects of the pure drugs, especially in the case of markedly cytotoxic drugs such as naproxen.